Abstract: Crustacean hyperglycemic hormone ( CHH) family plays an important role in the regulation of metamor-phosis development, reproduction, molting, blood glucose balance and internal osmotic pressure balance in crusta-
cean. CHH is an impoitant member of CHH family. In this study, the proximal promoter sequence of chh gene was cloned by Tail-PCR from the mud crab, Scylla paramamosain. The sequence is 657 bp starting from the translation start site ( ATG). Forecast analysis results by the bioinformatics software showed that the transcription start site is
located at 74 bp upstream of start codon ATG, and TATA box is located at 31 bp upstream of transcription start site. The core promoter region is located between -41 bp and +9 bp, including the forecasted transcription start site. Potential transcription factor binding sites include Oct-1, C/EBPalp, GATA-1, Spl, Ap-1, NF-KappaB, NF-1, RAP1, HNR3, and so on.There is no CpG island in the proximal promoter sequence of chh gene. Four dele
tion fragments of chh gene promoter Cl ( -583 ~ +205 bp), C2 ( -388 ~ +205 bp)，C3 ( -286 - +205 bp)and C4 ( -46 - + 205 bp) were cloned and their promoter activities were analyzed using pEGFP-1, pGL3-Basic or
 pIEx-Lue2 reporter constructs. Activity analysis of deletion fragments in HEK293T and Sf9 cells all showed that there are binding sites of potential negative transcription factors between -286 - 46 bp and -583-388 bp,and that there are binding sites of potential positive transcription factors between -388-286 bp.