Abstract: In order to investigate the characteristics and regional differences of macrobenthic communities in seagrass beds in South China Coast, a survey was carried out in 12 seagrass beds in South China Coast in 2020. A total of 33 sections and 99 sites were investigated and the species composition, density, biomass, dominant species and the diversity parameters of macrobenthic community were calculated. Then we use statistical methods such as correlation analysis, cluster analysis, nMDS analysis and similarity analysis to describe the characteristic of the macrobenthic community and its relationship with seagrass communities. A total of 199 species of macrobenthos from 9 phyla were identified, among which mollusk species accounted for 39.7%. The average density of benthos was 155.7 ind/m² with the lowest density in Qingge-longwan (Qionghai) and the highest in Zhenzhu Bay (Fangchenggang). The average biomass was 118.36 g/m² with the lowest biomass Yifeng River (Shantou) and the highest in Zhenzhu Bay (Fangchenggang). The dominant species of benthos were mainly gastropods, bivalves and polychaetes. The average species diversity index of benthos was 1.12 with the lowest in Tangjia Bay (Zhuhai) and the highest in Tieshan Port (Beihai). The most parameters of macrobenthic community showed generally higher in order of Beibu Gulf > Hainan coast > Pearl River Estuary and eastern Guangdong. The regional characteristics of macrobenthic communities of seagrass beds were obvious, and the characteristics of the macrobenthic community were similar in closer seagrass bed areas. The higher the seagrass density and total biomass, the higher the species diversity index. The community structure of macrobenthos in seagrass beds dominated by large and medium-sized seagrass was more complex than that in small seagrass beds.