Abstract: In order to clarify the species diversity of the toxic Pseudo-nitzschia in China and declare their domoic acid (DA) production, four monoclonal strains were established from the samples of Taiwan Strait during May, 2017. Based on the morphology under light and transmission electron microscopy, and molecular analysis inferred from internal transcribed spacer (ITS) region of rRNA encoding gene, as well as the analysis of ITS2-RNA secondary structure, a newly recorded Pseudo-nitzschia species of China was identified as P. lundholmiae. It is characterized by lanceolate valve, presence of central interspacer, striae comprising one row of poroids and poroids normally dividing into two sectors. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), DA was only detected from one strain, with an average concentration of 2.3 fg/cell.Artemia salina and Paracalanus crassirostris were selected to mix-culture with P. lundholmiae, to evaluate the impact of zooplanktons on the DA production. The result showed that the existence of zooplankton could increase the DA production of P. lundholmiae in 3-4 times. The above results add new knowledge to the diversity of the toxic Pseudo-nitzschia in China and provides the DA features of P. lundholmiae, which can provide the basic data for further studies.