Abstract: Karyotype is the foundation of fish genetics. As one of the important species for aquaculture， the karyotype study of Takifugu bimaculatus has little been reported. In this study， the metaphase chromosomes ofT. bimaculatus was obtained from head kidney tissue by the method of phytohemagglutinin （PHA） and colchicine injection， and then the karyotype was analyzed. The results show that edthatthere was of 44 chromosomes in the diploid and the karyotype formula was 12m+6sm+26t，NF=62. With results of comparison and analysis， it is suggested that 2n=44 is the chromosome karyotype of Takifugu. The highly conservative of chromosome karyotype may be the one main reason of easily interspecific hybridization in genus Takifugu.