Abstract: The diversity of culturable fungi derived from four coral species from the South China Sea were investigated in this study. By spread plate method, YM, PDA, Martin, CDA, RBM media were used to isolate coral-associated fungi. Identification and phylogenetic analysis of coral-associated fungi were conducted based on ITS-rRNA gene sequencing. A total of 120 fungi strains were isolated from 4 corals ( Platygyra sp., Goniopora sp., Acropora microphthalma and Acropora florid ) and these fungi strains belong to 11 genera, including Penicillum (56 strains), Aspergillus (25 strains), Acremonium (2 strains), Cladosporium (5 strains), Meyerozyma (14 strains), Peniophora (2 strains), Phomopsis (1 strain), Schizophyllum (1 strain), Sterigmatomyces (13 strains) and Talaromyces (1 strain). The most diverse coral species was Acropora microphthalma and Acropora floride, both including 6 genus and 10 species. Fungal species distribution varied among different coral species. Peniophora sp. and Schizophyllum commune were only recovered from the coral species Acropora microphthalma , while Sterigmatomyces sp. and Talaromyces verruculosus were only present in the coral species Goniopora sp.. Only one strainPhomopsis sp. was obtained from coral Platygyra sp. Total number and species of culturable fungal strains recovered from the five media. YM media recovered the highest amount of fungal strains, accounting for 29.17% of the total strains. CDA media recovered the most diverse fungal species. These results suggest that there are diverse fungal symbionts associated with corals collected from the South China Sea, which are important sources for marine bioactive compounds.