Abstract: To test the effects of immunestimulating complexes (BSA-ISCOMs）, which was prepared by bovine serum albumin (BSA), on the nonspecific immune responses of Anguillidae fish, European eels (Anguilla anguilla) were immunized intraperitoneally with BSA-ISCOMs or BSA, while the control group were injected with phosphate buffered solution (PBS). On the 30th day after the second immunization, serum samples were collected for non-specific immune parameters analysis, and the remaining eels were infected with Vibrio vulnificus and Aeromona hydrophila. The results showed that the eels immunized with BSA-ISCOMs presented significantly higher lysozyme activity than the eels injected with BSA or PBS (p ≤0.05). Superoxide dismutase (SOD) and complement component 3 (C3) activities of the BSAISCOMs group were not significantly different from those of the BSA or PBS group (p ＞0.05). Survival rates of the BSA-ISCOMs group were significantly higher than those of BSA or PBS group (p ≤0.05), with a relative percent survival (RPS) of 43.3% for V. vulnificus challenge and 51.7% for A. hydrophila challenge. This study revealed the potential benefit of BSA-ISCOMs for the immune responses and disease resistance of European eel.