| 摘要: |
| 以海带多糖提取率为评价指标，优化了微波复合酶辅助提取海带加工下脚料中多糖的最佳工艺条件。结果表明，海带废弃物原料为2.00 g时，微波辅助提取阶段的最佳工艺条件为：微波功率400 W、温度60 ℃、时间12 min、pH6.5和液料比60.00 mL/g，此工艺条件下海带多糖的提取率为106.49 g/kg；复合酶辅助提取阶段的最佳工艺条件为：酶解温度55 ℃、pH6.0、时间105 min、液料比40.00 mL/g、纤维素酶添加量4.00×103U、木瓜蛋白酶添加量4.00×103U、果胶酶添加量2.40×103U，在此工艺条件下海带多糖的提取率为115.16 g/kg。在上述微波复合酶辅助提取最佳工艺条件下，海带多糖的提取率可达 (208.93±0.18) g/kg。抗氧化试验测定结果表明，海带多糖具有一定的抗氧化活性，且清除羟自由基(·OH)的能力较强。 |
| 关键词: 海洋生物学 水产品加工 微波 复合酶 海带多糖 抗氧化性能 |
| DOI：10.3969/J.ISSN.2095-4972.2021.02.019 |
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| 基金项目:2016年山东省高等学校科技计划资助项目（J16LE60）；威海市特色果蔬高值加工工程技术研究中心科研专项资金资助项目（GSGC-2019-0002） |
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| Optimization of microwave and compound enzyme assisted extraction of laminaria polysaccharide and analysis of antioxidant activity |
| NIE Xiao-wei,CHEN Zhi-bin,GU Xiao-hui,WANG Xiao-lin,ZHANG Qin,REN Zhao-zhen,WU Qing-qing,WANG Lei |
| (College of Food Engineering, Weihai Ocean Vocational College, Weihai 264300, China) |
| Abstract: |
| Taken laminarin extraction rate as an index, the technological conditions of laminarin extraction from laminaria processing wastes were optimized by twostage method of microwave and compound enzyme. When taking laminaria raw material 2.00 g, the optimal technological conditions for the microwave stage were as follow: the microwave extraction solution was pH 6.5, the ratio of liquid to substrate was 60.00 mL/g with 12 min 400 W microwave treating at 60 ℃. Under these optimal conditions, the extraction rate of laminarin could reach 106.49 g/kg. The optimal technological conditions for the compound enzyme stage were as follow: the compound enzyme extraction solution was pH 6.00, the ratio of liquid to substrate was 40.00 mL/g and the enzyme treated for 105 min at 55 ℃ with the dosage of combined enzymes including cellulase 4.00×103U, papain 4.00×103U and pectinase 2.40×103U. Under these optimal conditions, the extraction rate of laminarin could reach 115.16 g/kg. Under the optimal conditions of the microwave and compound enzyme assisted extraction, the extraction rate of laminarin could reach (208.93±0.18) g/kg. The results of antioxidant activity showed that the extracted laminarin had certain antioxidant capacity besides a stronger scavenging activity of hydroxyl (·OH). |
| Key words: marine biology aquatic product processing microwave compound enzyme laminarin oxidation resistance |
