| 摘要: |
| 采集广西北部湾钦州附近月亮湾、钦州港和茅尾海海域海洋腐木，采用稀释法分离纯化得到32株产孢真菌，对真菌的菌落、孢子和菌丝形态进行研究，32株真菌可被分为５种真菌类型，其中第Ｉ、ＩＩ和ＩＩＩ种类型的真菌仅分离于月亮湾，第ＩＶ和Ｖ种类型在３个样品采集地均有分离．通过ＩＴＳ基因测序表明，３２株真菌为５个不同的种，其分别属于子囊菌门（Ａｓｃｏｍｙｃｏｔａ）和接合菌门（Ｚｙｇｏｍｙｃｏｔａ），可以确定种属的包括伞状霉属（Ｕｍｂｅｌｏｐｓｉｓ）和青霉菌属（Ｐｅｎｉｃｉｌｌｉｕｍ），其中分离的青霉菌属在数量上具有优势，占分离菌株总数的７５％，属于优势种属．采用４种指示菌大肠杆菌（Ｅｃｏｌｉ）、蜡样芽孢杆菌（Ｂａｃｉｌｌｕｓｃｅｒｅｕｓ）、烟草节杆菌（Ａｒｔｈｒｏｂａｃｔｅｒｎｉｃｏｔｉａｎａｅ）、副溶血弧菌（Ｖｉｂｒｉｏ ｐａｒａｈａｅｍｏｌｙｔｉｃｕｓ）检测抑菌活性，发现两株伞状霉属真菌Ｕｍｂｅｌｏｐｓｉｓｐ．Ｂ１０和Ｕｍｂｅｌｏｐｓｉｓｓｐ．Ｂ９菌丝体提取物对四种指示菌均具有较强的抑制作用，另外两株青霉属真菌Ｐｅｎｉｃｉｌｌｉｕｍｓｐ．Ａ５和Ｐｅｎｉｃｉｌｌｉｕｍｓｐ．Ｂ７的菌丝浸出物分别对革兰氏阳性细菌和革兰氏阴性副溶血弧菌具有较强的抑制活性．本研究为首次报道广西北部湾海洋腐木真菌及其抗菌活性． |
| 关键词: 海洋生物学 ＩＴＳ基因 分子鉴定 海洋腐木 产孢真菌 北部湾 |
| DOI： |
|
| 基金项目:国家自然科学基金资助项目（31560727）；广西自然科学基金资助项目（2014GXNSFBA118135）；钦州市科技开发资助项目（201322034）；广西北部湾海洋生物多样性养护重点实验室开放课题资助项（2015ZB02） |
|
| Isolation, identification and antibacterial activity of the spore produting marine rotten wood fungi from Beibu Gulf |
| GONG Bin,FANG Huaiyi,XIONG Zheng,YI Cuihua,LIAO Riquan,SONG Jingjing,ZHANG Yanqiu |
| (Ocean Department. Qinzhou University. Guangxi Key Laboratory of Beibu Gulf Marine ?Biodiversity Conservatian. Qinzhou 535000, China) |
| Abstract: |
| Wood-rotting fungi from ocean is an important source of novel fungal species and biologically active secondary metabolites. In our research, rotten woods from Yueliang Bay, Qinzhou harbour and Maowei sea of Beibu
Gulf were collected, and 32 fungi were isolated using gradient diluted separation method. Five fungi were identified based on characterization of fungal colonies, spores and some sporangia. Among them, three (morphological typeI、II and III ) were only isolated from Yueliang Bay, and the other two ( morphological type IV and V )were isolated from all the sampling sites. These fungi belonged to 5 taxa and 2 phylun ( Ascomycota and Zygomycota) according to ITS sequence and phylogenetic reconstruction assay and closely matching to the known Genus Penicillium, and Umbelopsis in GenBank Database, and the most abundant taxa were Penicillium ( account for 75% ). U-sing E. coli, Bacillus cereus、Arthrobacter nicotianae, Vibrio parahaemolyticus as testing strains. the extract from hypha of Umbelopsis sp. B10 and B9 showed strong inhibiting activity against these bacteria, and Penicillmm sp. A5
and B7 inhibited the growth of Gram-negative bacteria of the tested strains and Vibrio parahaemolyticus. Our research is the first report of wood-rotting fungi and their antibacterial activity from Beibu Gulf in China. |
| Key words: marine biology ITS gene molecular identification marine rotten wood spore producing fungi |
