| 摘要: |
| 最近报道从患病的红螯螯虾（Ｃｈｅｒａｘｑｕａｄｒｉｃａｒｉｎａｔｕｓ）中分离出一种新的虹彩病毒，被暂时命名为红螯螯虾虹彩病毒．它还能感染克氏原螯虾（Ｐｒｏｃａｍｂａｒｕｓｃｌａｒｋｉｉ）和凡纳滨对虾（Ｌｉｔｏｐｅｎａｅｕｓ ｖａｎｎａｍｅｉ），为了探究红螯螯虾虹彩病毒是否能够感染同属于甲壳纲、十足目的螃蟹．选取中华绒鳌蟹（Ｅｒｉｏｃｈｅｉｒｓｉｎｅｎｓｉｓ）和粗腿厚纹蟹（Ｐａｃｈｙｇｒａｐｓｕｓｃｒａｓｓｉｐｅｓ）作为实验对象，应用实时荧光定量ＰＣＲ技术和透射电子显微镜技术，结论证明了红螯螯虾虹彩病毒能够感染中华绒鳌蟹和粗腿厚纹蟹．推测其他蟹类也可能是其潜在的宿主，这暗示着红螯螯虾虹彩病毒对螃蟹的养殖是一种潜在的威胁． |
| 关键词: 海洋生物学 红螯螯虾虹彩病毒 中华绒鳌蟹 粗腿厚纹蟹 实时荧光定量PCR |
| DOI： |
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| 基金项目:国家自然科学基金资助项目（31272684）；江苏省自然科学基金资助项目（BK2012678）；福建省海洋高新产业发展专项资助项目（闽海洋高新［2014］05号） |
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| Study of Cherax quadricarinatus iridescent virus in two crabs |
| PAN Changkun,YUAN Huifang,WANG Tiantian,YANG Feng,CHEN Jianming |
| (School of Marine Sciencea, Ningbo University, Ningbo 315000, China;State Key Laboratory of Marine Genetic ?Resources, Third Institute of Oceanography, SOA.Xiamen 361005, China) |
| Abstract: |
| It is reported recently a new iridovirus, isolated from sick Cherax quadricarinatus, is temporarily named as Cherax quadricarinatus iridescent virus ( CQIV), which has infected Procambarus clarkii and Litopenaeus vannamei. In order to explore tfie capability of infection on crabs of Crustacea, Decapoda by the virus. Used Eriocheir sinensis and Pachygrapsus crassipes as the experimental object on which the real-time fluorescence quantitative poly-merase chain reaction ( PCR) and transmission electron microscopy technique is applied. It is concluded that the virus can infect Eriocheir sinensis and Pachygrapsus crassipes. We speculate that other crabs may also be the poten-tial host and it implies that CQIV poses a potential threat to the cultured crab. |
| Key words: marine biology Cherax quadricarinatus iridescent virus Eriocheir sinensis Pachygrapsus crassipes real-
time qPCR |
