Abstract: The embryonic development of Eleutheronema tetradactylum is studied in this paper. Zygotes fertilized naturally were obtained from 2 years old cultured E. tetradactylum during the processing of natural spawning. The result shows that mature zygote of E.tetradactylum is round with an oil ball in the center， the average diameter of zygote is 675.78μm， and the oil ball average diameter was 258.46μm. The embryonic development finished in 13 h 52 min under 31~33℃ at salinity 27.50～28.00 and pH 8.2. The process of embryonic development is fast which can be divided into 6 stages， including cell division stage， blastula stage， gastrula stage， embryo development stage， organs differentiation stage and hatching stage. Body length of newly hatched larvae is 1.46±0.25mm， while 3DAH larvae is 2.42±0.18mm with the mouth and anal opening， being able to feeding initiatively.